Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells

Standard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) test is recommended genotoxicity testing, but cytochalasin-B (Cyt-B) may interfere with nanoparticles (NP), leading to inaccurate results. Our objective was determine whether Cyt-B could MN induction by TiO2 NP human SH-SY5Y cells, as assessed CBMN test. Cells were treated 6 or 24 h, according three treatment options: co-treatment Cyt-B, post-treatment, and delayed co-treatment. Influence of on cellular uptake evaluated flow cytometry (FCMN) assessed. significantly internalized both the absence presence indicating that this chemical does uptake. Dose-dependent increases rates observed after However, FCMN assay only showed a positive response when added simultaneously NP, suggesting alter No differences comparisons between options assessed, they alternatives avoid interference specific conditions tested.